or has not enough money in my checking account?
No. According to USCIS, When they receives notice that your check has expired, they will notify you and suspend processing on your case. You will be given an opportunity to send a new check at the original filing rate and filing date to resume processing of your application. USCIS will contact you if your check has been returned to because it is beyond the expiration date or has not enough fund.
* This is just for your reference.